(30. The REF button has no valid meaning for fluorometric use, and if pressed when in fluorometric mode, will switch. SpectraMax M2 and M2e Multi-Mode Microplate Readers User Guide Molecular Devices SpectraMax M2 and M2e Multi-Mode Microplate Readers. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. With-dye, Continual Mode Assay on a SpectraMax M2 Plate Reader. Item Description FrontPanelOperation SinglewavelengthAbsorbance,%Transmittance,Fluorescence readingofthecuvette(ortesttube) UsingSoftMaxProSoftware ExpressdataasAbsorbance. . 1 SoftMax Pro v4. The method is based on. Dual monochromators for assay flexibility With SpectraMax M Series readers, there is no need to utilize. Ideal for a wide range of applications, including Western Blot analysis, this SpectraMax i3x includes the additional MiniMax 3000 Imaging Cytometer module for advanced cell imaging. type: Clear: Add to Cart. Molecular Devices SpectraMax™ M2e Microplate Reader w/ Warranty. • Flexible design for future expansion • User upgradable in < 2 minutes • High speed detection for up to 1536-well microplates • Excellent. 305 reference level saturation. Gemini XPS Fluorescence Microplate Reader . The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. Three kits covering a wide range of dsDNA concentrations. Measuring Time: Reading Times (Flying Mode 1 flash): 9 sec (96), 16 sec (384) Wavelength Range:SpectraMax 190: SpectraMax 340PC384: SpectraMax Plus384: VERSAmax: Vmax: Tecan: Sunrise: Trinean: DropSense96: AMBIENT TEMPERATURE STORAGE: Liconic: LPX 220 Hotel: LPX 440 Hotel: Thermo Fisher Scientific: Cytomat 10 Hotel: Cytomat 2 Hotel: Cytomat 24 Hotel: Cytomat 8 Linear Hotel: Cytomat 9 Hotel (MPH) BAR CODE. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. Reader Documentation Version No. 0 out of 5 $ 7,999. SpectraMax M5 is a fantastic plate reader - easily my #2 behind PerkinElmer (if you can afford them). Microplate Readers. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. Plate chamber design of the SpectraMax category 1 microplate readers. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. In addition, combined absorbance and fluorescence intensity assays can be run by issuing a single read command. S. Open and turn on the laptop. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard plate reader applications, protecting your initial investment. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Page 1 SpectraMax M2/M2 user guide ® A MULTI-DETECTION MICROPLATE READER WITH TWO-MODE CUVETTE PORT. 0 out of 5 $ 2,999-20%. read matrix, spectral scanning and detection of plate formats from 6- to 384-wells, the SpectraMax iD3 reader is the complete solution for all your research needs. 9000-0756. 0) OD (0. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Biotek Elx800 Microplate Reader. 0) OD (0. Main Menu Toggle. Perkin Elmer Envision. g. was 1. Microplate spectrophotometers, also called microplate readers, allow researchers to analyze multiple samples in parallel using 6- to 1536-well formats, as opposed to single sample measurements. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. Category 2 and 3 microplate readers use gradient heating as well. In the second assay, HepG2 cells were plated into a 96-well clear-bottom, blackwalled plate at 10,000 cells/well and incubated overnight at 37°C. Fluorescence Microplate Reader Comparison Chart. The Molecular Devices Gemini EM is built on the Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. You should use solid white plates for top Time- Resolved Fluorescence reads, and white plates with clear bottoms for bottom reads. Microplate Readers Include the M3, M4, M5, and M5. Specifications LUMINESCENCE PHOTOMETRIC PERFORMANCE Detection limit (384-well microplate) 10 amol/well alkaline phosphatase, 200 µL Wavelength range 250–850 nm TIME. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Fluorescence intensity values increased over time, but similar EC 50 values of 263 nM and 295 nM were obtained for assay incubation times ranging from one. Conclusions. In the M2, M5 and Plus 384, the light. D 59 A. It combines the sensitivities of filters with the convenience of a monochromator to provide the complete laboratory solution with absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. The plate reader optics are tunable for absorbance. Published Dec 16, 2020 | Updated Dec 16, 2020. When displaced, changes in fluorescence can be read in an FP, FI, or TR-FRET depending on the assay format. SpectraMax Quant dsDNA Assay Kits. Molecular Devices SpectraMax M2 ; Molecular Devices. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or. Fluorescence is measured at time zero and again after one-hour of incubation at 37°C in a fluorescence microplate reader. With optimized reagents, option to operate the readers. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Add to Cart. Test Plates for absorbance and fluorescence intensity provide a complete traceable solution for validating optical performance of the SpectraMax M2. Microplate Readers Biotek ELx808 Microplate Reader Absorbance. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. The SpectraMax® i3 Multi-Mode Detection Platform for absorbance, fluorescence, and luminescence detection, and the ability to add cartridges as new assays are developed. 53 - 60 kg, (117-132 lb), depending on configuration. Summary of Contents for Molecular Devices SpectraMax M2. 7°C (gradient heating design). In addition, the SpectraMax iD5Popular answers (1) If you are going to read the plate from the top, you should use a solid black plate. For problems with SpectraMax Multi-Mode Microplate Readers that are not listed here, in the U. 1 New Reader Support 1. 316 grating motor driver faulty. Wavelength optimization. Item Description Sensitivity 10amol/wellalkalinephosphate200µL Wavelengthrange 250-850nm Crosstalk <0. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v2. SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. Standard features include a cuvette port,. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. SpectraMax M5), place the adapter in the plate carriage. Microplate Readers. On the SpectraMax i3x reader, HTRF (cartridge) should be selected as the Optical Configuration. The data acquisition and analysis is easily streamlined when using the preconfigured protocol in Softmax Pro Sofware. degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner. Test Plates for absorbance and fluorescence intensity provide a complete traceable solution for validating optical performance of the SpectraMax M2 reader, automatically. Figure 2 shows a Lck kinase dilution curve obtained using the FAM-p34cdc2-derived peptide. It is also extremely flexible and can measure samples held in the 96, 384, 1536 and other. item 1 Molecular Devices SpectraMax M2 Multi-Mode Microplate Reader - JVSHJH O Molecular Devices SpectraMax M2 Multi-Mode Microplate Reader - JVSHJH O $4,999. The FlexStation 3 reader’s optics are designed to easily adapt to changes in assay requirements while maintaining the optimal performance of a single-mode reader. Data from slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. . Tecan’s. Fluorescence measurements were acquired with a Molecular Devices SpectraMax M2 Microplate Reader using 96 well plates, with the samples being excited at 468 nm and emission being measured at 572 nm. This reader's dual monochromators for. . 6 % of reading 4. 40266 Email: drugdiscoverytech@lifetech. VANTAstar™ Flexible Multi-mode Microplate Reader. Multi-Mode Microplate Readers including the M3, M4, M5, and M5. 1 cm) Weight 27 lb. 04 to 3. Detection Ranges: absorbance 200-1000nm, fluorescence Ex: 250-850nm / fluorescence Em: 360-850nm, luminescence: 360–850 nm. This can increase throughput and efficiency, thus saving time and space in the lab. 6% for the on-board optics (monochromators). SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. The Molecular Devices SpectraMax M2 Microplate Reader is a multidetection microplate reader with dualmonochromators, dual-mode cuvette ports, and top-reading capability. SoftMax Pro Software has been integrated by many leading robotics and LIMS partners, enabling both data analysis and. . 9000-0756. Absorbance cuvette port. fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400 750 nm for readings in fluorescence polarization mode (M5, M5 e models only). The high sensitivity, low-noise photomultiplier tubes (PMTs), and variable-volume. The kit includes everything you need: reagents, buffers and a microplate. 0 out of 5 $ 9,995. Highly selective for dsDNA over ssDNA or RNA. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. free tracer decreases, resulting in an overall increase in RFU values. 100 readings per well gives optimal Z’ values for this assay. The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). Therefore, ADP production is proportional to an increase in fluorescence. Specifications LUMINESCENCE PHOTOMETRIC PERFORMANCE Detection limit (384-well microplate) 10 amol/well alkaline phosphatase, 200 µL Wavelength range 250–850 nm TIME-RESOLVED FLUORESCENCE PERFORMANCE Summary of Contents for Molecular Devices SpectraMax M2. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. The plates were then read with the SpectraMax M3 plate reader (Molecular Devices, Sunnyvale, CA) to obtain absorbance readings at 570 nm. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). 8 is required to operate the new SpectraMax® M5 reader and its five read types: Fluorescence Intensity, Absorbance, Luminescence, Time-Resolved Fluorescence (TRF) and Fluorescence Polarization. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or spectrophotometer. Description The onboard microprocessor calculates and reports the absorbance, % transmittance, and/or fluorescence for each well of a microplate or from the cuvette port. Microplate Readers Molecular Devices Spectramax M5 Microplate Reader. M2. SpectraMax Paradigm SpectraMax i3 SpectraMax Flex-Station 3 SpectraMax F5 SpectraMax M4, M5, and M5e SpectraMax. Microplate Formats: 6, 12, 24, 48, 96, 384. A microplate reader is a device used to detect chemical, biological, or physical reactions by measuring emitted light. . Enables accurate fluorescence data with every run. Top reading usually provides better signal-to-noise ratios for solution-based assays such as DNA quantification or protein quantification. Temperature Control: ambient from +4 to 45C. 00. 2 % of reading SpectraMax Plus384 microplate reader and absorbance plate ABS1 SpectraMax ABS Plus microplate reader and Absorbance plate. Add to Cart. Shop now. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. In the M2, M5 and Plus. Tecan Infinite F200 Fluorescence Microplate Reader. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. 020 OD 2. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. 2 only) SpectraMax M-series Microplate readers. The SpectraDrop Micro-Volume microplate offers the ability to use as little as 2 µL samples with 24 or 64-well plates. The purple holder is used for reading non-transparent plates from the top (e. KEY FEATURES • Use the touchscreen to easily set up methods, runSpectraMax® i3xMulti-ModeMicroplateReader SpectraMax® i3Multi-ModeMicroplateReader FlexStation® 3Multi-ModeMicroplateReader SpectraMax® M5eMulti-ModeMicroplateReader SpectraMax®M5Multi-ModeMicroplateReader SpectraMax® M4Multi-ModeMicroplateReader SpectraMax® M3Multi. 5). 5°C at 37°C. 2. The Gemini EM can sample a variety of formats, from 6- to 384-well microplates, in different modes, such as endpoint, spectral scan, kinetic, and well scan. 0 out of 5 $ 16,499. Warranty: 60. 4. Usage/Application: Lab. The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. Our absorbance plate readers feature our PathCheck Sensor. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. The plate is set up for reading and images are acquired according to specified parameters. I am wondering if the type of 96-well plate matters. 6 % of reading 4. When using the PyroWave XM Reader, excitation wavelengths of 380 nm and emission wavelengths of 440 nm are used to measure fluorescence. The SpectraMax Paradigm Detection Cartridge design utilizes an energy intensity optimization technique that allows the user to read up to seven logs of sample concentration in a single pass of a plate without any manual intervention. patent nos. S. With exceptional signal-to-noise ratio and a lower detection limit. . Filters: Excitation and emission slides for 4 filters each. Multimode microplate readers offer flexibility, and ease of use. Manufacturer: Molecular Devices; Model: SpectraMax; The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2. Molecular devices SpectraMax M3 Pdf User Manuals. When the plate was read on the SpectraMax M5 microplate reader, an enzyme concentration range of 0. It is important to determine the excitation and emission wavelengths that provide the best dynamic range for the assay, since these may differ somewhat from those used with filter-based plate readers. The SpectraDrop Micro-Volume microplate offers the ability to use as little as 2 µL samples with 24 or 64-well plates. The SpectraTest fluorescence test plate (FL-1) can be used by Gemini XPS & M2 readers. A . This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. The SpectraMax i3x reader provides theThis Molecular Devices SpectraMax iD5 Microplate Reader is made in Oct. microplate readers provide flexibility, performance, and ease of use for a variety of microplate assays. Appendix A. For paper-based measurements, we used the Milenia HybriDetect 1 (TwistDx) lateral flow system, as per the manufacturer's. The SPECTRAmax GEMINI XS Microplate Spectrofluorometer from Molecular Devices is a microplate reader capable of detecting signal from either fluorescence or luminescence based assays. Our lab has a BMG labtech Pherastar FS, it is easy to use and we have never had any issue with it. 25 to 3. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well. A. both cuvette-based and microplate. The instrument is located in room 2131 at 300 George Street. 10. spr SpectraMax iD3 SpectraTest ABS2 v1. Figure 1. All SpectraMax® M series multi-mode microplate readers measure UV and visible absorbance, and fluorescence intensity measurements on microplates and cuvettes as standard. Prior to fluorescence analysis, the lid from the OmniTray plate was removed. spr SpectraMax iD3 SpectraTest ABS2 v1. Microplate Readers Include the M3, M4, M5, and M5. The excitation and the emission. 313 reference gain check fail. The data acquisition and analysis is easily streamlined when using the preconfigured protocol in Softmax Pro Sofware. Synergy H1 is a modular multimode microplate reader, with monochromator-based and filteru0002based optics. 0 software was used to handle data analysis and control each instrument. Description The onboard microprocessor calculates and reports the absorbance, % transmittance, and/or fluorescence for each well of a microplate or from the cuvette port. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Documentation:Plate readers that are used to detect luminescence, fluorescence and absorbance signals can vary in sensitivity (Figure 2). Fluorescence Microplate Readers 4. Optimized Settings to Validate the Molecular Devices SpectraMax® M2/M2e Microplate Readers with the Transcreener® Fluorescence Intensity Assays. 0) OD (500 to 1100) RFU (5. 87. From advanced multimode luminescence, fluorescence, absorbance, BRET and FRET capabilities to dedicated microplate or single-tube luminometers, there is a GloMax® system for every lab and. Thanks to HTRF signal stability, the assay plate may be used to check several instruments, or. When reading optical density at wavelengths below 340 nm, special UVtransparent,. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening. Standard 6-, 12-, 24-, 48-, 96- and 384-well microplates. These measurements can include parameters such as absorbance, fluorescence intensity and. Features. Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. The plate reader. The HTRF Reader Control Kit provides an easy and straightforward way to assess your reader’s overall performance and can facilitate the identification of potential limitations. 020 OD 2. ~ Bio-Tek ELx808 Microplate Reader $2,795. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. Third this recommendation. The fluorescence was measured in the SpectraMax M3 Microplate reader. SpectraMax M2E Multimode plate reader with cuvette option, ABS and FLR reading and extended range. SpectraMax Microplate Readers. The SpectraMax 190 reader can be integrated with Molecular . In simplest terms, a plate reader measures and records a signal produced from the wells of a multiwell plate. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. For example, microplates containing different samp les can be read using the same orThe SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. . 3: The cuvette chamber. 67. For example, microplates containing different samp les can be read using the same orThe plate is then covered and mixed to allow complete cell lysis before the luminescent signals are read on the SpectraMax i3x Multimode Microplate Reader (Figure 1). 验证检测 SpectraMax 190 的工作状态。 堆板机、机械臂一体化整合 SpectraMax 190 可以在几分钟内整合到 Molecular Devices 的 StakMax 堆板机上。为了实现更高程度的自动化，自动化供应商 合作伙伴已经把我们的酶标仪系统和所有主要合作伙伴的机械臂 进行了整合。I am interested in using a SpectraMax M2 Plate Reader for a spectrophotometric assay and am trying to collect the proper reagents and materials. 00. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter. The chamber contains. Published Dec 16, 2020 | Updated Dec 16, 2020. The Cut-off filter for this pair is showing 530nm in this machine. Category 2 and 3 microplate readers use gradient heating as well. 0077 OD 0. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. Bottom Monochromator: Fluorescein 2. The reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. 8 foot) 4400-0002 Power cord, 1 meter (3. This plate reader is capable of monitoring UV and visible absorbencies, fluorescence, and luminescence. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. min at 37°C. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. Microplate Readers. In the lower half of the window, left side, there is a box labeled reader. Fill a clean plate with water. Luminescence, and Fluorescence microplate re aders—providing extensive data calculation. Figure 2-23: Bottle holder To install the bottle holder: 1. A solution for low volume applications. 1) A maximal fluorescence intensity of 177 ± 3 RFU was observed at the highest cell concentration (Table 1). CA$14,500. Page 2 SpectraMax ® SpectraMax ® Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089. SpectraMax ABS and ABS Plus Absorbance ELISA Microplate Readers. Microplate Readers Molecular Devices SpectraMax M2 Multimode Plate Reader. Butyrate (5 μM, 24h), doxorubicin (1 μM, 2h) and H 2 O 2 (2 mM. 0 out of 5 $ 3,895. Molecular Devices SpectraMax M2 Microplate Reader. fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400–750 nm for readings in fluorescence polarization mode (M5, M5e models only). Assay plates containing the 15-point standard curve were read on the Molecular Devices' SpectraMaxš M2 Microplate Reader. The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. $16,000. Microplate Readers. Test Plates for absorbance and fluorescence intensity provide a complete traceable solution for validating optical performance of the SpectraMax M2 reader, automatically. E Models. When a. Results were graphed in SoftMax Pro Software using a 4-parameter curve fit. Analyze samples using fluorometry, photometry, luminometry, TRF or FP with these. Then the working solution (50μL) was added to the plate for 30 min treatment without light at room temperature and then the stop solution (50μL) was added. The following general steps are taken with our absorbance and fluorescence readers. Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. 00. Measure more accurately. 0 out of 5 $ 14,499 $ 13,995-13%. Step 2: Click SoftMax Pro’s Read button. 2018. The Gemini EM can sample a variety of formats, from 6- to 384-well microplates, in different modes, such as endpoint, spectral scan, kinetic, and well scan. SpectraMax M2 plate reader (Molecular Devices) was employed to measure the absorbance at 490 nm and 680 nm. SpectraMax M2 and M2e Performance Specifications SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. Incubation. Microplate Formats: 6, 12, 24, 48, 96, 384. Reviews. SpectraMax M3 Reader SpectraMax M4 Reader SpectraMax M5 Reader SpectraMax M5e Reader Absorbance ü ü ü ü Fluorescence Intensity ü ü ü ü Luminescence ü ü ü ü Time-Resolved Fluorescence ü ü ü Fluorescence Polarization ü ü HTRF ü Upgrade Options TRF, HTRF, FP HTRF, FP HTRF N/A Plate Formats 6-, 12-, 24-, 48-, 96-, 384. When samples are added to the wells, the Protein G molecules are resuspended and binding occurs. Assays requiring a read in two or more modes can be combined and run on the SpectraMax Multi-Mode Microplate Readers by issuing. 4. B. MOLECULAR DEVICES SPECTRA MAX GEMINI XS MICRO. SPECTRAmax GEMINI’s wavelength choices for cutoff filters are 420, 435, 455, 475, 495, 515, 530, 550, 570, 590, 610, 630, 665 and 695 nm. fluorescence intensity (Figure 6). Plate reader. Clamp for injector 1 is on the left and clamp for injector 2 is on the right. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v1. In addition, combined absorbance and fluorescence intensity assays can be run by issuing a single read command. 0 out of 5 $ 3,895. The SpectraMax® M2 and M2e Systems from Molecular Devices are multi- detection microplate readers with dual- monochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). • Fluorescence Intensity (FI) • Luminescence (Lum) • Time-Resolved Fluorescence (TRF) • Fluorescence Polarization (FP) The SpectraMax M5e reader offers the additional benefit of being certified for Cisbio Bioassays HTRF® technology. Instructions. The top- and bottom-reading Gemini EM Microplate Reader measures fluorescence on a variety of sample formats from 6- to 384-well microplates in endpoint, kinetic, spectral scan, and well-scan modes. . The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. Suggested plates for best data: Fluorescence-top read; Solid Black flat-bottom for Excitations above 350 nm;. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. ABS1 Absorbance Plates, FL1 Fluorescence Plates, and LM1 Luminescence Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. D 47 7. 0 Concentration Dynamic Range 105 RFU MT oltage ignal Adjustable PMT gain High. The dual monochromator optics allow the. Reads various kinds of 96-wells plates, and is equipped with shaking function. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Readers – Photometric Accuracy Fluorescence Intensity Luminescence Intensity (0. 0) OD (0. WinKQCL ® Software can be used with a compatible microplate reader to run the template and. Protein assays. 2. 5,112,134 5,766,875. 8: theMicroplate Reader & Luminometer Selection Guide. Plate Shaking. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). ID: MOLECULAR DEVICES SpectraMax M2 is a multi-mode microplate spectrophotometer providing unbeatable accuracy, dependability and versatility for any lab experiment, with a range of detection modes, advanced accessories, and intuitive software. Test Plates for absorbance and fluorescence intensity provide a complete traceable solution for validating optical performance of the SpectraMax M2 reader, automatically. 00 CAD. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. Page 2 SpectraMax ® SpectraMax ® Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part #0112-0102 Rev. We here describe a fluorescence resonance energy transfer (FRET) based fluorescence plate reader assay to monitor the interaction between fluorescently tagged sortilin and p75 NTR in live cells. CO 2 & O 2 control. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, SpectraMax M4 reader, SpectraMax M3. With optimized reagents and the industry leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax to perfectly match your needs. 0077 OD 0. Offer. SpectraMax Gemini EM Microplate Reader A dual-monochromator spectrofluorometer system Dual monochromators. Part Number Description 1-2100-0548 USB computer connection cable, 3 meter (9. Similar settings will work for other SpectraMax readers with fluorescence detection. The chamber.